{"id":505,"date":"2021-01-10T20:34:18","date_gmt":"2021-01-10T19:34:18","guid":{"rendered":"https:\/\/simona-huwiler.net\/?page_id=505"},"modified":"2026-04-21T23:49:02","modified_gmt":"2026-04-21T21:49:02","slug":"research","status":"publish","type":"page","link":"https:\/\/simona-huwiler.net\/index.php\/research\/","title":{"rendered":"Research"},"content":{"rendered":"\n
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Learning from predatory bacteria as a source of novel therapeutic agents: <\/mark><\/strong>
from \u2018omics\u2019 to molecular mechanisms<\/mark><\/strong><\/h3>\n\n\n\n
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As a way to tackle the antibiotic resistance crisis natural killers of bacteria like predatory bacteria have gained attention in the scientific community recently1<\/sup>. Gram-negative predatory bacterium B. bacteriovorus<\/em> has not only a big potential as a putative \u2018living antibiotic\u2019 by invading a wide range of Gram-negative (antibiotic resistant) pathogens in\/on different organisms2-8<\/sup>, but as well as a biotechnological tool9<\/sup> or as a source of potential new drugs due to its predatory and lytic capacity towards Gram-negative bacteria. B. bacteriovorus <\/em>is ubiquitously present in the environment10-18<\/sup> and a model organism for predatory bacteria that invade and replicate in the periplasm of other Gram-negative prey bacteria (Fig. 1)19<\/sup>. Once nutrients become scarce B. bacteriovorus<\/em> septates to produce progeny which escape the depleted host cell remnants in the exit phase20 <\/sup>(Fig. 1, highlighted in yellow).<\/p>\n\n\n

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Fig. 1. Predatory life cycle of B. bacteriovorus HD100.<\/strong> In the predatory life cycle predator B. bacteriovorus divides within the periplasm of the Gram-negative prey bacterium. In the exit phase juvenile prey cells leave the depleted prey cell.<\/figcaption><\/figure>\n<\/div>\n\n\n
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To date the molecular mechanisms underlying this last, crucial phase remain enigmatic apart from a novel predatory lysozyme that I characterized in collaboration with Prof. R. E. Sockett (University of Nottingham, UK) & Dr. A. L. Lovering (University of Birmingham, UK) during my SNSF Postdoc.Mobility fellowships21<\/sup>. This predatory lysozyme specifically recognizes and cleaves the nonself-marked (deacetylated GlcNAc) peptidoglycan in the prey bacterial cell wall as part of the prey exit and is potentially useful as a drug21<\/sup>. However, the prey exit mechanism involves degradation of other cellular structures with new predatory mechanisms to discover. I aim to learn from predatory bacteria with the help of state-of-the-art \u2018omics\u2019 technologies (transcriptomics, proteomics, metabolomics) and down to the detailed level of predatory molecular mechanisms. This knowledge is essential in order to use predatory bacteria or their novel enzymes in potential applications as biotechnological tools or therapeutic agents\/drugs.<\/p>\n\n\n\n

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